Wastewater treatment plants receiving inconsistent influent loads are often challenged with maintaining an effective biological treatment. Many of the key microorganisms in wastewater processes are dependent on organic substrate to grow and divide. When influent flows are limited, the biodegradable carbon source for the treatment system may be too low to sustain biomass. Not having enough organic substrate in the plant will diminish the biological treatment efficiency. Calculating the food to microbiology ratio (F:M) is one way to monitor changes in organic carbon quantity and biomass in a system. Although optimal F:Ms are variable among different plants, F:Ms will decrease below stable values when the carbonaceous food supply is limited.